Synopsis

DNA and RNA are built from just four nucleotide elements but rich in information content and functional diversity. DNA sequence encodes the cell's genetic blueprint and operating system, and must be copied with unprecedented fidelity once every cell cycle. The integrity of the genetic blueprint is vital to the cell and maintained by various mechanisms to fend off the constant onslaught of DNA damage. In addition to replication, DNA is repeatedly transcribed to RNA for protein synthesis (translation) and cellular regulation (non-coding RNA). Nevertheless, readout of DNA at single-nucleotide level by cellular machineries takes place in the context of the compacted DNA form (chromosomes), within which 2 m of human genomic DNA are compacted and stored in the cell nucleus.

In contrast to the relatively uniform DNA double helix, RNA assumes versatile structure and function. RNA is the worker bee of genetics to the DNA's queen. In addition to its roles as messenger (mRNA), interpreter and amino acid-carrier (tRNA), RNA is also the critical functional part of the ribosome and spliceosome machines and can catalyze peptide bond formation and phosphoryl-transfer reactions (and who knows what more?) as ribozymes. Furthermore, RNA can act as a molecular switch responding to small molecules (riboswitches) to control gene expression or mediating and executing DNA and RNA cleavage as in the CRISPR-Cas defense system. Failure of any of these DNA and RNA transactions can lead to disease. Meanwhile application of antisense oligonucleotide (ASO) and CRISPR-Cas technology have revolutionized gene therapy, and cell and organism manipulation.

Key Sessions

In the 8^th nucleic acid conference, the central theme will be to understand processes involving DNA and RNA at the molecular and chemical level, with a marked structural and mechanistic perspective. We plan to have an up-to-date discussion of the following: 

•    DNA replication in the context of chromatin and telomere
•    DNA repair and recombination
•    Transcription and splicing
•    RNA structure and function
•    Translation and post-translation modification
•.   Gene editing by ASO and CRISPR-Cas9 
•    AI-assisted nucleic acid research 

Confirmed Invited Speakers 

Valérie Borde (Curie Institute)
SINGLE MOLECULE ANALYSES OF RECOMBINATION-ASSOCIATED DNA SYNTHESIS
Yiliang Ding (John Innes Centre)
RNA STRUCTURE, AN IMPORTANT REGULATOR IN LIVING CELLS
Sebastian Fica (Oxford University)
MAKING mRNAs: ATPase REMODELLING AND SPLICE SITE SELECTION IN THE HUMAN SPLICEOSOME
Yang Gao (Rice University)
BIOCHEMICAL PROFILING AND STRUCTURAL BASIS OF ADAR1-MEDIATED RNA EDITING
Eric Greene (Columbia University)
SINGLE MOLECULE STUDIES OF DNA RECOMBINATION 
Alba Guarné (McGill University)
TARGET-SITE SELECTION MECHANISMS OF THE Tn7 TRANSPOSON
Nick Guydosh (NIH)
Taekjip Ha (Harvard University)
Yuan He (Johns Hopkins University)
STRUCTURAL BASIS OF DNA DOUBLE-STRAND BREAK REPAIR BY NHEJ
Leemor Joshua-Tor (Cold Spring Harbor Lab)
MAD ABOUT U: REGULATING THE let-7 miRNA PATHWAY
Ailong Ke (Yale University)
LEVERAGING MECHANISTIC INSIGHTS FOR CRISPR TOOL DEVELOPMENT
Elizabeth Kellogg (St. Jude's Childrens Research Hospital)
UNDERSTANDING AND ENGINEERING TRANSPOSONS FOR GENOME-EDITING
Jeffrey Kieft (New York Structual Biology Center)
NEW METHODS, CHALLENGES, AND FRONTIERS IN RNA STRUCTURAL BIOLOGY
Ci Ji Lim (University of Wisconsin-Madison)
NEW INSIGHTS INTO HUMAN TELOMERE MAINTENANCE MACHINERY
Giulia Palermo (University of California)
COMPUTATIONAL GENE EDITING
Lori Passmore (MRC)
MECHANISTIC INSIGHTS INTO THE POLY(A) TAIL MACHINERY
Titia Sixma (Netherlands Cancer Institute)
STRUCTURAL STUDIES IN MMR ACTIVATION
Song Tan (Penn State University)
HOW CHROMATIN PROTEINS AND ENZYMES RECOGNISE THE NUCLEOSOME
Keith Weninger (North Carolina State University)
MISMATCH REPAIR PROTEIN IMPACTS ON ATYPICAL TRINUCLEOTIDE REPEAT DNA CONFIGURATIONS
Steve West (Francis Crick Institute)
THE TWO RAD51 PARALOG COMPLEXES – CHANGING PARADIGMS
Eric Westhof (Université de Strasbourg)
GEOMETRIC RECOGNITION AND RNA MODIFICATIONS IN TRANSLATION
Cynthia Wolberger (Johns Hopkins Medical School)
HISTONE UBIQUITINATION IN TRANSCRIPTION REGULATION
Sarah Woodson (Johns Hopkins University)
STICK SLIP UNFOLDING AND AGGREGATION OF TRIPLET REPEAT RNA
Wan Yue (Genome Institute of Singapore)
Yuanliang Zhai (HKUST)
STRUCTURE AND FUNCTION OF EUKARYOTIC REPLISOME
Jinwei Zhang (NIH)
RECOGNITION ND MIMICRY OF THE tRNA FOLD
Xiaodong Zhang (Imperial College London/ Francis Crick Institute)
HOW A MOLECULAR MOTOR OPENS UP DOUBLE-STRANDED DNA FOR TRANSCRIPTION
Xiaolan Zhao (Memorial Sloan Kettering Cancer Center)
SMC-BASED REGULATION OF DNA REPAIR