Programme
WEDNESDAY 21ST FEBRUARY |
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14:00 – 15:00 |
Registration & Reception |
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14:00 – 15:00 |
Welcome Lunch |
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15:00 – 15:15 |
Opening Comments |
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DNA Recombination |
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15:15 – 16:00 |
Steve Kowalczykowski |
SINGLE-MOLECULE VISUALIZATION OF PROTEIN-DNA COMPLEXES: UNDERSTANDING BIOLOGY, ONE MOLECULE AT A TIME |
16:00 – 16:30 |
Eli Rothenberg |
SINGLE-MOLECULE STUDIES OF HUMAN DNA DOUBLE STRAND BREAK REPAIR PATHWAYS IN VITRO AND IN CELLS |
16:30 – 17:00 |
Jie Yan |
LABEL-FREE QUANTIFICATION OF SEQUENCE AND STRUCTURE SPECIFIC DNA-PROTEIN INTERACTIONS |
17:00 – 17:30 |
Refreshments |
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17:30 – 18:00 |
Jie Xiao |
MULTISTABLE CELL FATE DECISION - A SINGLE-MOLECULE STUDY OF GENE REGULATION |
18:00 – 18:30 |
Joseph Loparo |
VISUALIZING DNA BREAK REPAIR: HOW THE NHEJ MACHINERY MINIMIZES MISTAKES |
18:30 – 18:45 |
Sharonda LeBlanc |
THE DYNAMICS OF MISMATCH REPAIR INITIATION |
18:45 – 19:00 |
Kata Sarlós University of Copenhagen |
SINGLE-MOLECULE AND BIOCHEMICAL RECONSTITUTION OF ANAPHASE DNA BRIDGE DISJUNCTION
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19:00 – 19:30 |
Piero Bianco |
INSIGHT INTO STALLED DNA REPLICATION FORK RESCUE IN E.COLI |
19:30 |
Dinner at Leisure |
THURSDAY 22ND FEBRUARY |
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07:00 – 08:30 |
Breakfast *Kalmia Buffet Restaurant* |
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Chromosome Dynamics Tim Lohman |
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08:30 – 09:15 |
Dave Sherratt |
THE ACTION OF SINGLE MukBEF SMC COMPLEXES IN LIVE E. COLI CELLS |
09:15 – 09:30 |
Hong Wang |
COHESIN SA1 AND SA2 RECOGNIZE SPECIFIC DNA SEQUENCES AND STRUCTURES: A TALE OF TWO PROTEINS
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09:30 – 10:00 |
Sunney Xie |
3D GENOME STRUCTURES OF SINGLE DIPLOID HUMAN CELLS |
10:00 – 10:30 |
Refreshments |
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10:30 – 11:00 |
Terence Strick |
CORRELATIVE SINGLE-MOLECULE ANALYSIS OF DNA REPAIR SYSTEMS |
11:00 – 11:30 |
Long Cai |
SPATIAL GENOMICS AND SINGLE CELL LINEAGE DYNAMICS BY SEQFISH AND MEMOIR |
11:30 – 12:00 |
Johan Elf |
CHARACTERIZING A LIBRARY OF CELLS AT SINGLE MOLECULE SENSITIVITY |
12:00 – 16:30 |
Lunch at Leisure & Free Time |
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12:30 – 16:00 |
Snorkel Trip (Sign-ups required in advance) |
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DNA Replication |
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16:30 – 17:00 |
Nick Dixon |
THE BACTERIAL REPLISOME – DESIGN PRINCIPLES FOR A DYNAMIC MOLECULAR MACHINE |
17:00 – 17:30 |
Mike O’Donnell |
OPTICAL TRAPS, FLUORESCENT PROTEINS AND MICROFLUIDICS REVEAL UNEXPECTED DYNAMICS OF EUKARYOTIC CMG HELICASE |
17:30 – 18:00 |
Kiyoshi Mizuuchi |
INTRACELLULAR LONG-RANGE COMMUNICATION IN BACTERIAL PLASMID SEGREGATION AND SEPTUM POSITIONING |
18:00 – 18:30 |
Refreshments |
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18:30 – 19:00 |
Rodrigo Reyes Lamothe |
REPLISOME DYNAMICS IN LIVE BACTERIA AND BUDDING YEAST |
19:00 – 19:30 |
Samir Hamdan |
SINGLE MOLECULE VIEW OF THE STRUCTURE SPECIFIC 5’ NUCLEASES |
19:30 – 19:45 |
Enrico Monachino |
SINGLE-MOLECULE AND BULK BIOPHYSICAL STUDIES OF ESCHERICHIA COLI DNA REPLICATION REVEAL A CONFORMATIONAL SWITCH CONTROLLING REPLISOME STABILITY |
19:45 – 20:00 |
Lisanne Spenkelink |
SINGLE-MOLECULE VISUALIZATION OF YEAST LEADING-STRAND SYNTHESIS REVEALS DYNAMIC INTERACTION BETWEEN MTC AND THE REPLISOME |
20:00 |
Dinner at Leisure |
FRIDAY 23RD FEBRUARY |
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07:00 – 08:30 |
Breakfast *Kalmia Buffet Restaurant* |
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DNA Repair |
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08:30 – 09:15 |
Yuri Lyubchenko |
DYNAMICS OF PROTEIN-DNA COMPLEXES AT NANOSCALE |
09:15 – 09:30 |
Andrew Robinson |
SINGLE-MOLECULE IMAGING OF TRANSLESION DNA POLYMERASES IN E. COLI |
09:30 – 10:00 |
Mark Leake |
PROBING GENE CONTROL IN LIVE CELLS USING HIGH-SPEED SINGLE-MOLECULE FLUORESCENCE MICROSCOPY |
10:00 – 10:30 |
Refreshments |
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10:30 – 11:00 |
Stephan Uphoff |
ILLUMINATING THE REGULATION OF BACTERIAL DNA REPAIR AND MUTAGENESIS USING SINGLE-MOLECULE AND SINGLE-CELL MICROSCOPY |
11:00 – 11:30 |
Myron Goodman |
A SINGLE MOLECULE WINDOW INTO ATP REGULATION OF THE DNA POLYMERASE V MUTASOME |
11:30 – 12:00 |
Tim Lohman |
LARGE DOMAIN MOVEMENTS REGULATE UvrD HELICASE ACTIVITY |
12:00 – 16:00 |
Lunch at Leisure & Free Time |
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Genomics |
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16:00 – 16:45 |
Fuchou Tang |
SINGLE-CELL MULTI-OMICS SEQUENCING OF MOUSE EARLY EMBRYOS AND EMBRYONIC STEM CELLS |
16:45 – 17:00 |
Manal Zaher |
MISSED CLEAVAGE OPPORTUNITIES BY FEN1 LEAD TO OKAZAKI FRAGMENT MATURATION VIA THE LONG-FLAP PATHWAY |
17:00 – 17:30 |
Will Greenleaf |
SINGLE-CELL EPIGENOMICS MAPS THE CONTINUOUS REGULATORY LANDSCAPE OF HUMAN HEMATOPOIETIC DIFFERENTIATION |
17:30 – 18:00 |
Refreshments |
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18:00 – 18:15 |
Wei Ting Chelsea Lee |
SPATIOTEMPORAL ASSOCIATIONS OF DNA G4 AND REPLICATION IN HUMAN CELLS |
18:15 – 19:00 |
Peter Fraser |
3D CHROMOSOME AND GENOME DYNAMICS |
19:00 – 19:30 |
Linda Kenney |
CHARGED RESIDUES IN THE H-NS LINKER DRIVE DNA BINDING AND GENE SILENCING IN SINGLE CELLS |
20:00 |
*Gala Dinner* |
SATURDAY 24TH FEBRUARY |
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07:00 – 08:30 |
Breakfast *Kalmia Buffet Restaurant* |
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Transcription |
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08:30 – 09:15 |
Steve Block |
RIBOSWITCH FOLDING, STRUCTURE AND FUNCTION |
09:15 – 09:45 |
Richard Ebright |
REPURPOSING NANOPORE DNA SEQUENCING TO ANALYZE RNA POLYMERASE TRANSLOCATION WITH SUB-NUCLEOTIDE, SUB-MILLISECOND RESOLUTION |
09:45 – 10:15 |
Eric Galburt |
DNA UNWINDING AT A DISTANCE DURING EUKARYOTIC TRANSCRIPTION INITIATION |
10:15 – 10:45 |
Refreshments |
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10:45 – 11:30 |
Achillefs Kapanidis |
UNCOVERING TRANSCRIPTION MECHANISMS VIA SINGLE-MOLECULE FLUORESCENCE IMAGING |
11:30 – 12:00 |
Sabrina Leslie |
HOW BIOMOLECULES BEHAVE IN A SQUEEZE |
12:00 – 12:15 |
Avin Ramaiya |
QUICKER THAN QUICK? CORRELATIVE FLUORESCENCE-FORCE MICROSCOPY AS A VERSATILE TOOL FOR DNA-MECHANICS |
12:15 – 12:35 |
Panel Discussion |
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12:35 – 12:45 |
Closing Comments |