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- 2nd DNA & Interacting Proteins as Single Molecules - In Vitro and In Vivo Conference
2nd DNA & Interacting Proteins as Single Molecules - In Vitro and In Vivo Conference
DNA Replication, Recombination, Repair and Transcription at the single molecule-level #DIPSM20
20 Feb - 23 Feb 2020
Nassau, Bahamas
Early Bird - Expired • Talk Submission - Expired • Poster Submission - Expired • Registration & Payment Deadline - Expired
Report
Please follow the link below to read the Editorial from the 2nd DNA and Interacting Proteins as Single Molecules - In Vitro and In Vivo 2020 Conference.
https://academic.oup.com/nar/article/49/11/6005/6307326?login=true
Synopsis
The advent of single-molecule approaches has revolutionized our understanding of Molecular Biology. Combined advances in light microscopy, microfluidics, atomic force microscopy, and DNA sequencing have opened ways to bypass population averages and study individual molecular events for a variety of cellular processes.
This meeting aims to present the most recent advances in the mechanistic understanding of DNA metabolic processes at the single-molecule level, using both in vivo and in vitro approaches.
Sessions will cover various aspects of:
- DNA replication
- DNA recombination
- DNA repair
- Transcription
- Chromosome dynamics
- Genomics
Each session will be divided into in vitro and in vivo sections to highlight differences and complementary information obtained.
Student Offer
Take advantage of this fantastic opportunity for students! Register an academic at the full rate and bring a student for only $850. Unfortunately, Postdocs are not eligible for this offer. Both registration packages include; accommodation for the 20, 21, 22 February 2020 (on a shared basis for students) and a 24hour all-inclusive food and beverage package for the conference period. Once registered, please contact Emily Meen to obtain a special registration link for your student.
Confirmed Plenary Speakers
Vincent Croquette (l’ESPCI Paris)
Dorothy Erie (University of North Carolina at Chapel Hill)
INTEGRATIVE SINGLE-MOLECULE STUDIES OF DNA MISMATCH REPAIR INIATION
Antoine van Oijen (University of Wollongong)
SINGLE-MOLECULE STUDIES OF EUKARYOTIC DNA REPLICATION
Maria Spies (The University of Iowa)
MOLECULAR CHOREOGRAPHY OF THE RPA-DNA-RAD52 COMPLEX DURING DNA REPLICATION, RECOMBINATION AND REPAIR
Confirmed Invited Speakers
Ibrahim Cisse (Massachusetts Institute of Technology)
SUPER-RESOLUTION IMAGING OF TRANSCRIPTION IN LIVE MAMMALIAN CELLS
Johan Elf (Uppsala University)
LIVE CELL IMAGING OF DOUBLE STRANDED BREAK REPAIR REVEALS FAST HOMOLOGY SEARCH
Ilya Finkelstein (University of Texas at Austin)
COHESIN ORGANIZES THE GENOME VIA LOOP EXTRUSION
Roberto Galletto (Washington University in St.Louis)
ROLE OF PIF1-HELICASES AT HARD-TO-REPLICATE SITES
Samir Hamdan (King Abdullah University of Science and Technology)
BUILDING THE REACTION TIMELINE OF FEN1 DURING LAGGING STRAND DNA SYNTHESIS
Rogelio Hernández (synmikro)
UNDERSTANDING PROTEIN MOBILITY IN BACILLUS SUBTILIS BY TRACKING SINGLE MOLECULES
Achillefs Kapanidis (University of Oxford)
DISSECTING TRANSCRIPTION MECHANISMS VIA REAL-TIME IMAGING OF RNA POLYMERASE CONFORMATIONAL CHANGES
Tatiana Karpova (NCI-NIH, LRBGE, OMC)
SINGLE-MOLECULE STUDIES OF TRANSCRIPTION OF CUP1 LOCUS IN YEAST SACCHAROMYCES
Steve Kowalczykowski (University of California, Davis)
WIN, LOOSE, OR DRAW: IMAGING ENCOUNTERS OF INDIVIDUAL MOTOR PROTEINS WITH NUCLEOSOME ARRAYS
Kiyoshi Mizuuchi (NIH)
TWO TYPES OF ParA/B/S PLASMID/CHROMOSOME PARTITION SYSTEMS IN BACTERIA: TWO DIFFERENT PARTITION COMPLEX ARCHITECTURES, A COMMON MECHANISM?
Keir Neuman (NIH)
HOMOLOGY SENSING VIA NON-LINEAR AMPLIFICATION OF SEQUENCE DEPENDENT PAUSING BY RECQ HELICASE
Alexandros Pertsinidis (Memorial Sloan Kettering Cancer Center)
SINGLE-MOLECULE STUDIES OF TRANSCRIPTION MECHANISMS
Xiaojun Ren (University of Colorado Denver)
VISUALIZATION OF EPIGENETIC PcG COMPLEXES BY USING LIVE-CELL SINGLE-MOLECULE IMAGING
Andrew Robinson (University of Wollongong)
DNA POLYMERASE IV IN DOUBLE STRAND BREAK REPAIR
Ralph Seidel (Universität Leipzig)
DISSECTING THE TARGET RECOGNITION BY CRISPR-CAS COMPLEXES USING SINGLE-MOLECULE NANOMECHANICAL MEASUREMENTS
Dave Sherratt (Oxford University)
ORGANIZATION OF THE ESCHERICHIA COLI CHROMOSOME BY A MukBEF AXIAL CORE
Terence Strick (Institut de Biologie de l'École Normale Supérieure)
SINGLE-MOLECULE ANALYSIS OF DNA REPAIR AND MUTAGENESIS
Gijs Wuite (Vrije Universiteit Amsterdam)
SINGLE MOLECULE MANIPULATION AND IMAGING OF COMPLEX DNA-PROTEIN TRANSACTIONS
Jie Xiao (John Hopkins School of Medicine)
Target Audience
Researchers interested in the study of DNA metabolic events at the single molecule level: both in vitro and in vivo. Processes to be focused on – DNA replication, DNA recombination, DNA repair and transcription. In addition, single cell sequencing/genomics groups will also be targeted.
Educational Need
The single molecule field initially focused on experiments done in vitro. As the field has evolved and new technologies were developed, the understanding of single molecule behaviour in live cells has become increasingly important. The two fields have developed largely separately and by combining these into a single meeting will educate scientists on the value of and difficulties associated with both approaches to understanding key elements in genome maintenance, and the passage of genetic information from DNA to protein.
Confirmed Speakers
Chairs
Plenary Speakers
Dorothy Erie
Professor, University of North Carolina
Antoine van Oijen
Distinguished Professor, University of Wollongong
Maria Spies
Professor of Biochemistry and Molecular Biology, University of Iowa
Vincent Croquette
Researcher, LPENS-CNRS / ESPCI
Invited Speakers
Steve Kowalczykowski
Professor, University of California, Davis
Samir Hamdan
Assistant Professor of Bioscience, King Abdullah University of Science and Technology
Gijs Wuite
prof, Vrije universiteit amsterdam
Johan Elf
Professor, Uppsala University
Xiaojun Ren
Professor, University of Colorado Denver
Kiyoshi Mizuuchi
Section Chief, National Institutes of Health
Ralf Seidel
Professor, University Leipzig
Keir Neuman
Senior Investigator, National Institutes of Health
Achillefs Kapanidis
Professor of Biological Physics, University of Oxford
Ilya Finkelstein
Associate Professor, UT-Austin
Rogelio Hernandez Tamayo
Postdoc, Philipps-Universität Marburg
Tatiana Karpova
Core Head, NCI-NIH, LRBGE, OMC
Ibrahim Cisse
Associate Professor, MIT
David Sherratt
Professor and group head, University of Oxford
Terence Strick
Professeur, Ecole normale supérieure
Jie Xiao
Professor, Johns Hopkins School of Medicine
Andrew Robinson
Research Fellow, University of Wollongong
Alexandros Pertsinidis
Associate Member, Memorial Sloan Kettering Cancer Center
Roberto Galletto
Associate Professor, Washington University in St. Louis School of Medicine
Programme
THURSDAY 20TH FEBRUARY |
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13:00 – 14:00 |
Student Networking Lunch |
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15:20 – 16:20 |
Registration & Welcome Reception |
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Transcription |
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16:20 – 16:30 |
Opening Comments |
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16:30 – 16:55 |
Achillefs Kapanidis |
DISSECTING TRANSCRIPTION MECHANISMS VIA REAL-TIME IMAGING OF RNA POLYMERASE CONFORMATIONAL CHANGES |
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16:55 – 17:10 |
Emiel Visser |
OBSERVING SINGLE MOLECULE PROTEIN-DNA INTERACTIONS AND DNA TRANSCRIPTION IN-VITRO USING TETHERED PARTICLE MOTION |
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17:10 – 17:35 |
Ibrahim Cisse |
SUPER-RESOLUTION IMAGING OF TRANSCRIPTION IN LIVE MAMMALIAN CELLS |
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17:35 – 18:20 |
Refreshments |
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18:20 – 18:35 |
Neil Kad |
THE TFIIH COMPONENTS P44/P62 ACT AS A DAMAGE SENSOR DURING NUCLEOTIDE EXCISION REPAIR |
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18:35 – 19:00 |
Alexandros Pertsinidis |
SINGLE-MOLECULE STUDIES OF TRANSCRIPTION MECHANISMS |
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19:00 – 19:15 |
Huan Zheng |
UNDERSTANDING THE CELL-SIZE REGULATION OF TRANSCRIPTION IN LIVE-YEAST USING SINGLE-MOLECULE TECHNIQUES |
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19:15 – 19:30 |
Harshad Ghodke |
MOLECULAR COUPLING BETWEEN TRANSCRIPTION AND DNA REPAIR MACHINERY IN LIVE CELLS DURING TRANSCRIPTIONAL STRESS |
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20:00 |
Dinner |
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FRIDAY 21ST FEBRUARY |
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07:00 – 09:00 |
Breakfast at Leisure |
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DNA Repair |
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09:00 – 09:35 |
Vincent Croquette |
KINETIC STUDIES OF DEAD AND DEAH HELICASES AND THEIR COFACTORS IN SINGLE MOLECULE ASSAY |
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09:35 – 09:50 |
Sunbok Jang |
WATCHING COOPERATIVE INTERACTIONS BETWEEN BASE AND NUCLEOTIDE EXCISION REPAIR PROTEINS |
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09:50 – 10:25 |
Dorothy Erie |
INTEGRATIVE SINGLE-MOLECULE STUDIES OF DNA MISMATCH REPAIR INIATION |
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10:25 – 10:50 |
Roberto Galletto |
ROLE OF PIF1-HELICASES AT HARD-TO-REPLICATE SITES |
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10:50 – 11:15 |
Kevin Raney |
TRANSLOCATION AND DNA UNWINDING ACTIVITIES OF PIF1 FAMILY HELICASES |
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11:15 – 12:50 |
Poster Session |
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12:50 – 16:50 |
Lunch at Leisure & Free Time |
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Recombination |
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16:50 – 17:25 |
Maria Spies |
MOLECULAR CHOREOGRAPHY OF THE RPA-DNA-RAD52 COMPLEX DURING DNA REPLICATION, RECOMBINATION AND REPAIR |
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17:25 – 17:50 |
Ilya Finkelstein |
COHESIN ORGANIZES THE GENOME VIA LOOP EXTRUSION |
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17:50 – 18:05 |
Alexandra Moores |
INVESTIGATING BACTERIAL NUCLEOTIDE EXCISION REPAIR IN MAINTAINING THE INTEGRITY OF REPLICATION FORKS USING SINGLE MOLECULE LIVE CELL IMAGING |
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18:05 – 18:50 |
Refreshments & Poster Viewing |
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18:50 – 19:15 |
Steve Kowalczykowski |
WIN, LOOSE, OR DRAW: IMAGING ENCOUNTERS OF INDIVIDUAL MOTOR PROTEINS WITH NUCLEOSOME ARRAYS |
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19:15 – 19:40 |
Gijs Wuite |
SINGLE MOLECULE MANIPULATION AND IMAGING OF COMPLEX DNA-PROTEIN TRANSACTIONS |
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19:40 – 20:05 |
Keir Neuman |
HOMOLOGY SENSING VIA NON-LINEAR AMPLIFICATION OF SEQUENCE DEPENDENT PAUSING BY RECQ HELICASE |
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20:30 |
Dinner |
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SATURDAY 22ND FEBRUARY |
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07:00 – 09:00 |
Breakfast at Leisure |
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Chromosome Dynamics I |
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09:00 – 09:35 |
Anton van Oijen |
SINGLE-MOLECULE STUDIES OF EUKARYOTIC DNA REPLICATION |
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09:35 – 09:50 |
Vlad-Stefan Raducanu |
ON DEMAND FLUORESCENCE MODULATION (ENHANCEMENT, QUENCHING OR NO EFFECT) FOR STUDYING PROTEIN-DNA INTERACTIONS |
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09:50 – 10:15 |
Xiaojun Ren |
VISUALIZATION OF EPIGENETIC PcG COMPLEXES BY USING LIVE-CELL SINGLE-MOLECULE IMAGING |
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10:15 – 11:00 |
Group Photo, Refreshments & Poster Viewing |
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11:00 – 11:25 |
Kiyoshi Mizuuchi |
TWO TYPES OF ParA/B/S PLASMID/CHROMOSOME PARTITION SYSTEMS IN BACTERIA: TWO DIFFERENT PARTITION COMPLEX ARCHITECTURES, A COMMON MECHANISM? |
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11:25 – 11:50 |
David Sherratt |
ORGANIZATION OF THE ESCHERICHIA COLI CHROMOSOME BY A MukBEF AXIAL CORE |
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11:50 – 12:05 |
Celia Viridiana Lopez |
STUDYING DNaA FUNCTION IN VIVO AT A SINGLE-MOLECULE LEVEL |
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12:05 – 16:25 |
Lunch at Leisure & Free Time |
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Chromosome dynamics II |
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16:25 – 16:50 |
Jie Xiao |
SPATIAL ORGANIZATION OF TRANSCRIPTION AND CHROMOSOME |
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16:50 – 17:05 |
Gurleen Kaur |
ENZYME DYNAMICS DURING LESION BYPASS AT THE E. COLI REPLICATION FORK |
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17:05 – 17:30 |
Johan Elf |
LIVE CELL IMAGING OF DOUBLE STRANDED BREAK REPAIR REVEALS FAST HOMOLOGY SEARCH |
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17:30 – 17:45 |
Yujing Ouyang |
DNA REPLICATION FORK ARREST BY ESCHERICHIA COLI TUS-TER INVOLVES MECHANISMS THAT ARE INDEPENDENT OF THE C6-MOUSETRAP MODEL |
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17:45 – 18:30 |
Refreshments & Poster Viewing |
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18:30 – 18:55 |
Rogelio Hernandez Tamayo |
UNDERSTANDING PROTEIN MOBILITY IN BACILLUS SUBTILIS BY TRACKING SINGLE MOLECULES |
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18:55 – 19:20 |
Tatiana Karpova |
SINGLE-MOLECULE STUDIES OF TRANSCRIPTION OF CUP1 LOCUS IN YEAST SACCHAROMYCES |
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19:20 – 19:45 |
Terence Strick |
Single-molecule analysis of DNA repair and mutagenesis |
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19:45 – 20:00 |
Kyle Vrtis |
PASSIVE AND ACTIVE REPLISOME UNLOADING DURING REPLICATION FORK COLLAPSE |
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20:00 |
Gala Dinner & Poster Awards |
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SUNDAY 23RD FEBRUARY |
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07:00 – 08:30 |
Breakfast at Leisure |
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DNA Replication |
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08:30 – 08:55 |
Samir Hamdan |
BUILDING THE REACTION TIMELINE OF FEN1 DURING LAGGING STRAND DNA SYNTHESIS |
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08:55 – 09:20 |
Rodrigo Reyes |
SUBUNIT DYNAMICS IN THE EUKARYOTIC REPLISOME IN LIVE CELLS |
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09:20 – 09:45 |
Ralf Seidel |
DISSECTING THE TARGET RECOGNITION BY CRISPR-CAS COMPLEXES USING SINGLE-MOLECULE NANOMECHANICAL MEASUREMENTS |
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09:45 – 10:15 |
Refreshments |
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10:15 – 10:40 |
Andrew Robinson |
SINGLE-MOLECULE LIVE-CELL IMAGING REVEALS RECB-DEPENDENT FUNCTION OF DNA POLYMERASE IV IN DOUBLE STRAND BREAK REPAIR |
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10:40 – 10:55 |
Karl Duderstadt |
VISUALIZATION OF TRANSCRIPTION-REPLICATION CONFLICTS REVEALS DYNAMIC REPOSITIONING OF MCM2-7 HELICASES PRIOR TO REPLICATION |
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10:55 – 11:10 |
Rohit Agarwal |
FLOW MAGNETIC TWEEZERS: IMAGING RARE ENZYMATIC EVENTS WITH SINGLE-MOLECULE PRECISION |
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11:10 – 11:20 |
Closing Comments |
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Supported by
Venue & Location
Melia Nassau Beach All Inclusive
Overlooking one of the finest beaches in the world the Meliá Nassau Beach is surrounded by crystal blue waters and white sandy beaches. Located on the stunning Cable Beach, you are just a few minutes walk from the center, shops and restaurants. The beautiful Nassau Botanical Gardens are within easy reach (7km) and Lynden Pindling International Airport is situated only 9km away.
Throughout your stay delegates will enjoy a full meal plan, inclusive of beverages. Take your pick from Cilantro where you can experience the natural textures, aromas and flavors of Mexico, Nikkei, celebrated for its exquisite fusion of Japanese, Cantonese and Peruvian cuisine featuring a sushi bar and Teppanyaki tables, Estavida, an upbeat tapas lounge located in the hotel lobby serving innovative tapas and a wide selection of cocktails, Aqua, an A la carte restaurant specializing in exquisite, rustic Italian cuisine, The Market Place, where you will find an International buffet serving an array of exquisite dishes, O'Grille, an open air restaurant with fabulous views of the sea and pool serving casual beach fare and light bites and finally The Black Angus, a steakhouse featuring hip, contemporary jazz and modern decor, offering guests the finest quality prime cuts and the freshest ingredients.(This restaurant is not included in the all inclusive package, additional charges apply). The Gala Night takes place on the third evening of the conference with a mouth-watering feast of local cuisine, an open bar and amazing local entertainment. We welcome all delegates and their accompanying persons to the Gala Night – a truly fun filled night not to be missed!
Hotel Facilities
- 24 hour reception
- Room service
- 3 heated outdoor pools
- Complimentary resort wide Wi-Fi (guest rooms, throughout hotel, beach and conference areas)
- 24-hour Fitness Center
- 7 dining venues
- 4 bars.
- non-motorised water sports
The Bahamas is formed by over 700 islands, keys and islets located in the Atlantic Ocean and renowned for its warm sunshine, mild climate, fine, white sandy beaches, turquoise, crystal clear water and friendly people, making this the perfect environment to relax and unwind in your free time during the conference.
General Information
Venue Rating
★ ★ ★ ★
Currency
US Dollar (USD)
Address
Nassau W Bay St. Nassau Bahamas
Nearest Airport
Lynden Pindling International Airport
Location
The Melia Nassau Beach All-Inclusive is located right on the stunning Cable Beach just a few minutes away from the airport and city center; Nearby in Downtown Nassau you can experience the flavours of new foods at local restaurants, shop 'til you drop at Straw market or take a trip through time at the Pirates Museum. The beautiful Nassau Botanical Gardens are close by and water enthusiasts can enjoy non-motorized water sports right on property.
Nassau is the capital of the Bahamas. It lies on the island of New Providence, with neighboring Paradise Island accessible via Nassau Harbor bridges. The city has a hilly landscape and is known for it's fabulous beaches as well as its offshore coral reefs, popular for diving and snorkeling. It retains many of its typical pastel-colored British colonial buildings, like the pink-hued Government House.
If you are interested in this meeting but not yet ready to register, you can sign up for updates here and our team will keep you updated regarding deadline reminders and grant opportunities relating to this meeting only.
Bronze Sponsors
Media Partners
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Conference Manager
Emily Meen
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